Gann Analysis Unveiled
Even though Gann was thought to be "religious", a careful analysis of his writings finds that he did not agree with the conventional Christian teachings. On page 99 to 100 of his novel The Tunnel Thru the Air (1927), Gann revealed some of his personal beliefs towards religion through a conversation between the protagonist, Robert Gordon, and his mother:
Gann Analysis Unveiled
We found some modules were remarkably associated with new tumor events, biochemical recurrence, pathological T, pathological N, and clinical stage, particularly for purple, red, and salmon modules. We found the red, purple, salmon, pink, magenta, midnight blue, grey60, and brown modules were significantly associated with RFS. The results of survival analysis based on these modules further indicated their biological significance, particularly as prognostic markers for PCa patients. Based on the importance of these modules, we chose purple and red modules as representatives to investigate their functional classification. KEGG enrichment analyses showed that these genes were mostly enriched in Cell Cycle and Aldarate Metabolism pathways, supporting their significant role in tumor development, consistent with the GSEA results.
Hub-gene analyses found that KIF23, PARM1, DONSON, MCM3, PCCA, and MTL5 played a critical role in connecting with other genes in the purple module, while PILRβ, FAM156A, and AHSA2 genes were strongly connected with other genes in the red module. We also tested the predictive values of these top positive/negative hub genes and found most of them displayed moderate predictive ability. Furthermore, based on the importance of these genes, we validated their expression in our own clinical samples. Before that, we confirmed the prognostic role of these genes as markers for RFS based on an online database and found the PILRβ was consistent with the module analysis, and that it is significantly associated with RFS of PCa patients. Therefore, we validated the PILRβ expression in our own clinical samples and found it was significantly higher in the high-risk tissues compared with the low-/middle-risk PCa tissues. Unfortunately, due to lack of follow-up information (we collected the clinical samples for less than 1 year, and the case number was also limited), we did not perform survival analysis in our cohort.
The rod and cone photoreceptors in the retina are highly specialized neurons that capture photons under dim and bright light, respectively. Loss of rod photoreceptors is an early clinical manifestation in most retinal neurodegenerative diseases that eventually result in cone cell death and blindness. The transcription factor NRL is a key regulator of rod photoreceptor cell fate and gene expression. Here, we report an integrated analysis of the global transcriptional targets of NRL. We have discovered that both NRL and CRX binding sites are present in genes involved in photoreceptor function, implying their close synergistic relationship. In vivo loss-of-function analysis of 16 NRL target genes in the mouse retina resulted in death or abnormal morphology of photoreceptor cells. Furthermore, we identified histone demethylase Kdm5b as a secondary node in the NRL-centered gene regulatory network. Our studies identify NRL target genes as excellent candidates for mutation screening of patients with retinal degenerative diseases, and they provide the foundation for elucidating regulation of rod homeostasis and targets for therapeutic intervention in diseases involving photoreceptor dysfunction.
Funding: This research was supported by intramural programs of the National Eye Institute, National Institute of Neurological Disorders and Stroke, and National Heart, Lung and Blood Institute, of the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Conceived and designed the experiments: AS HH DSK. Performed the experiments: HH DSK KC NG JG LG. Analyzed the data: HH DSK BK KRJ CZ NG AS. Contributed reagents/materials/analysis tools: BK KC CZ WP YF MS KZ. Wrote the paper: HH DSK BK AS.
1. The long-term maintenance of metabolism of representative drugs and steroid hormone substrates by cytochromes P-450, and their inducibility, was investigated in primary cultures of adult rat hepatocytes. Collagenase-isolated cells were seeded on collagen-coated tissue culture dishes and cultured in Chee's essential media in the presence or absence of phenobarbital (PB, 0.75 mM, 96 h or continuously) and 3-methylcholanthrene (MC, 5 microM, 48 h) for up to 45 days. 2. Hepatic P-450-dependent metabolism of diazepam to its primary oxidized metabolite was inducible by PB both in vivo (monitored in isolated liver microsomes) and in cultured cells (up to 100% and 400% increases in the formation of temazepam and nordiazepam, respectively, after 25 days in culture). Hepatocyte microsomal androstenedione 16 beta-hydroxylase activity was also induced by PB treatment of the hepatocytes (350-650% increase in 20-day-old cells). 3. Western blot analysis revealed that immunoreactive P-450 form PB-4 (IIB1), which catalysed the N-demethylation of diazepam to yield nordiazepam as well as androstenedione 16 beta-hydroxylation when assayed in a purified enzyme system, was substantially elevated following PB treatment of the cultured cells. Similarly, MC induced 7-ethoxycoumarin O-deethylase activity (up to 2000% increase from 5 to 45 days) as well as immunoreactive P-450c (IA1) in the hepatocyte cultures. 4. These studies demonstrate that cytochrome P-450 activities can be maintained, and also induced, after extended periods of time in hepatocytes cultured using a simple collagen mixture as substrate and a commercially available tissue culture media. This culture system should provide an important tool for further studies of P-450-dependent xenobiotic metabolism in a well-defined, liver-derived cellular system.
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It is unclear how wealthy W.D. Gann became from his trading analysis. In his 1993 book Trading for a Living, Alexander Elder, argues that Gann and his followers sold books and investment courses to earn money and did not profit from investments in the market. Elder's book claims that when W.D. Gann died in the 1950s his estate was valued at slightly over $100,000."}},"@type": "Question","name": "What Evidence Exists of W.D. Gann's Success?","acceptedAnswer": "@type": "Answer","text": "In 1909, Richard Wyckoff a reporter for Ticker and Investment Digest cited Gann's activity during October 1909. Gann made 286 transactions in various stocks, on both the long and short side of the market of which 264 of these transactions resulted in profits.","@type": "Question","name": "What Are the Tenets of W.D. Gann's Trading Philosophy?","acceptedAnswer": "@type": "Answer","text": "Gann encouraged his students to avoid overtrading and evaluate if a trade is based on hope or logic. He also taught followers to develop a different strategy for each of the four market situations: bull market; bull to bear market phase; bear market; and bear to bull market phase."]}]}] EducationGeneralDictionaryEconomicsCorporate FinanceRoth IRAStocksMutual FundsETFs401(k)Investing/TradingInvesting EssentialsFundamental AnalysisPortfolio ManagementTrading EssentialsTechnical AnalysisRisk ManagementNewsCompany NewsMarkets NewsCryptocurrency NewsPersonal Finance NewsEconomic NewsGovernment NewsSimulatorYour MoneyPersonal FinanceWealth ManagementBudgeting/SavingBankingCredit CardsHome OwnershipRetirement PlanningTaxesInsuranceReviews & RatingsBest Online BrokersBest Savings AccountsBest Home WarrantiesBest Credit CardsBest Personal LoansBest Student LoansBest Life InsuranceBest Auto InsuranceAdvisorsYour PracticePractice ManagementFinancial Advisor CareersInvestopedia 100Wealth ManagementPortfolio ConstructionFinancial PlanningAcademyPopular CoursesInvesting for BeginnersBecome a Day TraderTrading for BeginnersTechnical AnalysisCourses by TopicAll CoursesTrading CoursesInvesting CoursesFinancial Professional CoursesSubmitTable of ContentsExpandTable of ContentsEarly Life and EducationPublicationsGann AnglesCycles and NumbersW.D. Gann FAQsThe Bottom LineBusinessBusiness LeadersWho Was W.D. Gann?ByDan Blystone Full Bio LinkedIn Twitter Dan Blystone is the founder and editor of TradersLog.com, as well as the founder of the Chicago Traders Meetup Group.Learn about our editorial policiesUpdated December 06, 2022Reviewed byAkhilesh GantiW.D. Gann is remembered for using geometry, astrology, and ancient mathematics to predict events in the financial markets. He developed the technical analysis methods of Gann angles, indicators, and master charts.
It is unclear how wealthy W.D. Gann became from his trading analysis. In his 1993 book Trading for a Living, Alexander Elder, argues that Gann and his followers sold books and investment courses to earn money and did not profit from investments in the market. Elder's book claims that when W.D. Gann died in the